The 2-Minute Rule for cytometers
The 2-Minute Rule for cytometers
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Knowledge-enriched characterization of cell populations. Morphological data from images can include to the richness of flow cytometry data. Such as, the figure displays an if not standard apoptosis assay utilizing Annexin V and PI, incorporating mobile imaging to characterize cells in Every populace to expose morphologically unique attributes. These insights could not have already been received from multiplex staining alone.
Regardless of whether you’re a rookie or an authority, discover our resources to progress your investigation and abilities in flow cytometry.
Merge acoustic concentrating know-how with high-parameter Evaluation for versatile spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating by using a high-velocity brightfield digital camera for simultaneous substantial throughput flow cytometry and high resolution brightfield imaging.
"A picture isn't really pretty really worth 1000 dots, but it really actually helps you to see pictures. It is excellent to determine photos that correlate with staining. Such as, AnnexinV-constructive cells which have been morphologically distinctive than balanced cells, or CD14+ monocytes which might be larger sized and even more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a seasoned consumer has gated singlets confidently. Just after evaluating the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate consists of over 4% aggregates.
Optimizing gating strategy. Even sturdy manual singlet gating is error-prone and stays a subjective conclusion stage in Pretty much all flow cytometry assays. Imaging can be used to substantiate and change gates to incorporate only solitary cells of interest.
Our scientists will design panels in your case as again and again you may need—cost-free, no product or service order necessary.
Early signs of this contamination had originally been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also allows you to use morphological options to find exciting subpopulations that may not be apparent from flow cytometry data on your own.
Potentially most significantly, these situations consist of cells of Obviously unique phenotypes which could lead on to incorrect conclusions with regards to double constructive functions (particularly in exceptional populations).
AI-driven automatic impression analysis with the Attune Cytometric Software interprets occasion features into distinct morphology parameters which might be coupled with conventional fluorescence and scatter parameters to expose really reproducible populace adjustments and exceptional subsets.
Software Observe: Automatic graphic Investigation minimizes person-to-user variability in flow cytometry gating strategies
Leverage the power of Highly developed technology and innovation to speed up your discoveries. Have confidence in from the Attune Flow Cytometer family members to deliver the reliability, velocity, and precision that set your perform aside.
No wash/no lyse leukocyte Investigation with imaging. Cells were being obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Expert UX/UI permits people to educate AI models for expanded purposes. (A) The “Train Model” element provides annotation applications and authentic-time product coaching precision.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating engineering, which makes use of seem waves to meticulously align cells inside the flow mobile. This revolutionary tactic surpasses common hydrodynamic techniques, leading to speedier processing speeds, Extraordinary info high-quality, and increased sample throughput.
The Attune Xenith cytometer is often a significant-parameter spectral analyzer, supplying each spectral unmixing and conventional flow abilities for extensive and versatile cell Assessment.
The Attune CytPix Flow Cytometer makes it possible for end users to visualise samples in the course of acquisition, verify the accuracy of gating methods, and aid results with trusted documentation for publication reasons.
Listed here, we present that by gating methods using graphic-dependent quantitative parameters can distinguish interacting cells from coincident activities to much more accurately review interacting website cells.